Work made at Carnegie institution for Science, FZJ & HHU.
Background: I am a biologist and biophysicist, and I utilize molecular biophysics as a tool to gain knowledge about plant-microbe interactions. Understanding how plants and microbes interact with each other is a key question that needs to be addressed to achieve sustainability, especially in agriculture and within the context of the climate emergency.
Key question: how to monitor ions & molecules with high spatial and temporal resolutions in plants, microbes & plant-microbe systems.
Publications:
Differential regulation of glucose transport activity in yeast by specific cAMP signatures.
Clara Bermejo, Farzad Haerizadeh, Mayuri Sadoine, Diane Chermak, Wolf B. Frommer. Biochemical Journal, Portland Press Ltd., 2013, pp. 489—497
Selective double-labeling of cell-free synthesized proteins for more accurate smFRET studies.
Mayuri Sadoine, Michele Cerminara, Noémie Kempf, Michael Gerrits, Jörg Fitter, Alexandros Katranidis. Analytical chemistry, ACS Publications, 2017, pp. 11278--11285
Cotranslational incorporation into proteins of a fluorophore suitable for smFRET studies.
Mayuri Sadoine, Michele Cerminara, Michael Gerrits, Jörg Fitter, Alexandros Katranidis. ACS synthetic biology, ACS Publications, 2018, pp. 405—411
Preparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies.
Mayuri Sadoine, Michele Cerminara, Jörg Fitter, Alexandros Katranidis. Bio-protocol, 2018, pp. e2881--e2881
Affinity Purification of GO-Matryoshka Biosensors from E. coli for Quantitative Ratiometric Fluorescence Analyses.
Mayuri Sadoine$, Vanessa Castro-Rodriguez, Tobias Poloczek, Helene Javot, Erdem Sunal, Michael M Wudick, Wolf B Frommer. Bio-protocol, 2020, pp. e3773--e3773 ($corresponding author)
Affinity Series of Genetically Encoded Förster Resonance Energy-Transfer Sensors for Sucrose.
Mayuri Sadoine, Mira Reger, Ka Man Wong, Wolf B Frommer. ACS sensors, ACS Publications, 2021
Sensors for the quantification, localization and analysis of the dynamics of plant hormones. Reika Isoda, Akira Yoshinari, Yuuma Ishikawa,
Mayuri Sadoine, Rüdiger Simon, Wolf B Frommer, Masayoshi Nakamura. The Plant Journal, Wiley Online Library, 2021, pp. 542--557
Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.
Mayuri Sadoine, Yuuma Ishikawa, Thomas J Kleist, Michael M Wudick, Masayoshi Nakamura, Guido Grossmann, Wolf B Frommer, Cheng-Hsun Ho. Plant Physiology, Oxford Academic Journals, 2021
Monitoring nutrients in plants with sensors:Â Achievements and perspectives.
Mayuri Sadoine$, Roberto De Michele, Milan Župunski, Guido Grossmann, Vanessa Castro-RodrÃguez* (Plant Physiology)
From tracking protein conformational change to transport dynamics with using click chemistry methods.
Mayuri Sadoine*, Kambiz Hamadani*, Jörg Fitter$, Alexandros Katranidis* (in preparation)
A monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes shift fluorescent protein domain. J. Obinna Ejike*, Mayuri Sadoine*, Yi Shen*, Yuuma Ishikawa*, Erdem Sunal, Sebastian Hänsch, Anna Hamacher, Wolf B. Frommer$, Michael M. Wudick, Robert Campbell$, Thomas Kleist (ACS Biochemistry)
Genetically encoded biosensors reveal unique stress-induced shoot-to-root calcium signatures in Arabidopsis thaliana-Bacillus rhizobacteria interactions. Mayuri Sadoine*, Milan Župunski, Christian Frederick Kaiser, Clelia Lolito, Réjane Carron, Tonni Grube Andersen, Guido Grossmann* (in preparation)Â
Background: I am a biologist and biophysicist, and I utilize molecular biophysics as a tool to gain knowledge about plant-microbe interactions. Understanding how plants and microbes interact with each other is a key question that needs to be addressed to achieve sustainability, especially in agriculture and within the context of the climate emergency.
Key question: how to monitor ions & molecules with high spatial and temporal resolutions in plants, microbes & plant-microbe systems.
Publications:
Differential regulation of glucose transport activity in yeast by specific cAMP signatures.
Clara Bermejo, Farzad Haerizadeh, Mayuri Sadoine, Diane Chermak, Wolf B. Frommer. Biochemical Journal, Portland Press Ltd., 2013, pp. 489—497
Selective double-labeling of cell-free synthesized proteins for more accurate smFRET studies.
Mayuri Sadoine, Michele Cerminara, Noémie Kempf, Michael Gerrits, Jörg Fitter, Alexandros Katranidis. Analytical chemistry, ACS Publications, 2017, pp. 11278--11285
Cotranslational incorporation into proteins of a fluorophore suitable for smFRET studies.
Mayuri Sadoine, Michele Cerminara, Michael Gerrits, Jörg Fitter, Alexandros Katranidis. ACS synthetic biology, ACS Publications, 2018, pp. 405—411
Preparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies.
Mayuri Sadoine, Michele Cerminara, Jörg Fitter, Alexandros Katranidis. Bio-protocol, 2018, pp. e2881--e2881
Affinity Purification of GO-Matryoshka Biosensors from E. coli for Quantitative Ratiometric Fluorescence Analyses.
Mayuri Sadoine$, Vanessa Castro-Rodriguez, Tobias Poloczek, Helene Javot, Erdem Sunal, Michael M Wudick, Wolf B Frommer. Bio-protocol, 2020, pp. e3773--e3773 ($corresponding author)
Affinity Series of Genetically Encoded Förster Resonance Energy-Transfer Sensors for Sucrose.
Mayuri Sadoine, Mira Reger, Ka Man Wong, Wolf B Frommer. ACS sensors, ACS Publications, 2021
Sensors for the quantification, localization and analysis of the dynamics of plant hormones. Reika Isoda, Akira Yoshinari, Yuuma Ishikawa,
Mayuri Sadoine, Rüdiger Simon, Wolf B Frommer, Masayoshi Nakamura. The Plant Journal, Wiley Online Library, 2021, pp. 542--557
Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.
Mayuri Sadoine, Yuuma Ishikawa, Thomas J Kleist, Michael M Wudick, Masayoshi Nakamura, Guido Grossmann, Wolf B Frommer, Cheng-Hsun Ho. Plant Physiology, Oxford Academic Journals, 2021
Monitoring nutrients in plants with sensors:Â Achievements and perspectives.
Mayuri Sadoine$, Roberto De Michele, Milan Župunski, Guido Grossmann, Vanessa Castro-RodrÃguez* (Plant Physiology)
From tracking protein conformational change to transport dynamics with using click chemistry methods.
Mayuri Sadoine*, Kambiz Hamadani*, Jörg Fitter$, Alexandros Katranidis* (in preparation)
A monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes shift fluorescent protein domain. J. Obinna Ejike*, Mayuri Sadoine*, Yi Shen*, Yuuma Ishikawa*, Erdem Sunal, Sebastian Hänsch, Anna Hamacher, Wolf B. Frommer$, Michael M. Wudick, Robert Campbell$, Thomas Kleist (ACS Biochemistry)
Genetically encoded biosensors reveal unique stress-induced shoot-to-root calcium signatures in Arabidopsis thaliana-Bacillus rhizobacteria interactions. Mayuri Sadoine*, Milan Župunski, Christian Frederick Kaiser, Clelia Lolito, Réjane Carron, Tonni Grube Andersen, Guido Grossmann* (in preparation)Â
Comparative analysis of Bacillus reveals distinct regulation of bacterial motility in response to salt stress. Mayuri Sadoine*, Christian-Frederick Kaiser, Celine Weippert, Julia Engelhorn, Bruno Huettel, Ilka Bischoff, Thomas Hartwig*, Guido Grossmann* (in preparation)Â