Mayuri Sadoine

Biophysicist & Microbiologist Making biosensors for studying plant-microbe systems


Curriculum vitae



"I never lose. I either win or learn" -Nelson Mandela


Department of Biology (Institute of Cell and Interaction Biology)

Heinrich Heine Universität

Universitätstrasse 1, 40225 Düsseldorf (Germany)



Mayuri Sadoine

Biophysicist & Microbiologist Making biosensors for studying plant-microbe systems


Contact

Mayuri Sadoine

Biophysicist & Microbiologist Making biosensors for studying plant-microbe systems


Curriculum vitae



"I never lose. I either win or learn" -Nelson Mandela


Department of Biology (Institute of Cell and Interaction Biology)

Heinrich Heine Universität

Universitätstrasse 1, 40225 Düsseldorf (Germany)




About me


During my PhD in Juelich Research Center and my postdoc at the Institute for Molecular Physiology in HHU, I have acquired a strong expertise in molecular biophysics and the engineering of genetically encoded fluorophore-based biosensors which, once expressed in a specific organism, allow to measure steady-state levels of an analyte of interest in vivo. In many ways, biosensors are extraordinary tools which can be applied for addressing numerous biological questions such as how a cell feeds and responds to environmental changes.
I am getting a closer look at bacterial plant-pathogens. I am particularly interested in understanding how it is interacting with the host. In my research, we based our hypotheses on the postulate that pathogens infect hosts to multiply. In the case of a bacterial pathogen, multiplication means in fine nutrition. Thus, I am interested in how those unicellular organisms get nutrients for reproduction and take advantages of a very particular environment i.e., the host. In many cases, survival of the pathogen and its multiplication rely on smart and fine-tuned manipulations of the molecular machineries of the host and strategies of the infectious agent to take the lead of a race in which it needs to stay ahead of the host defense.
The necessity to understand host-pathogen interactions required to be able to enter into the system as an observer. This is as fascinating as challenging. In that purpose, I am expressing in the pathogen cytosol biosensors which respond to changes in steady-state levels of molecules related to nutrient transport and metabolism. The goal is to get new clues about how pathogens feed in planta and which molecular mechanisms and strategies are making them successful and virulent.

Investigate how a pathogen interacts with the host and its findings are actually relevant for any pathogen. E.g., numerous evolutionary mechanisms associated to virulence are similar to a broad range of infectious agents since driven by their particular life style which involved specific pressures of selection related to a necessity to co-evolve with the host while staying always one run ahead of its defense system and thus hidden to it. It is in a way very analogue to how a thief would need to act to be successful in robing valuable items from housing while not being detected by the alarm system.
Those last years, I have been working on projects related to sadly well-known  pathogens which might be responsible of epidemiological situations in crops in countries which eventually rely on those crops for feeding and economy. Consequent lost in crop yield are very damaging to the local population and in extreme circumstances can lead to severe public health related issues such as hunger situations. The research in the field is therefore shaped by a strong necessity to understand the molecular mechanisms underlying the disease with the need to keep a science at two speeds; one for providing short-term solutions to crisis period (e.g., providing new tools to farmers) and one for bringing long-term sustainability in resistant crops with bringing new knowledge in what make a pathogen more or less virulent which is the only way for us to be able to anticipate the emergence of new strains in taking lessons from past epidemiological situations.                         

Publications


OzTracs: Optical Osmolality Reporters Engineered from Mechanosensitive Ion Channels


Thomas J. Kleist, I Winnie Lin, Sophia Xu, Grigory Maksaev, Mayuri Sadoine, Elizabeth S. Haswell, Wolf B. Frommer, Michael M. Wudick


Biomolecules, vol. 12(6), 2022, p. 787


Sensors for the quantification, localization and analysis of the dynamics of plant hormones


Reika Isoda, Akira Yoshinari, Yuuma Ishikawa, Mayuri Sadoine, Rüdiger Simon, Wolf B Frommer, Masayoshi Nakamura


The Plant Journal, vol. 105, Wiley Online Library, 2021, pp. 542--557


Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology


Mayuri Sadoine, Yuuma Ishikawa, Thomas J Kleist, Michael M Wudick, Masayoshi Nakamura, Guido Grossmann, Wolf B Frommer, Cheng-Hsun Ho


Plant Physiology, Oxford Academic Journals, 2021


Sucrose-dependence of sugar uptake, quorum sensing and virulence of the rice blight pathogen Xanthomonas oryzae pv. oryzae


Mayuri Sadoine, Juying Long, Congfeng Song, Yugander Arra, Wolf B Frommer, Bing Yang


bioRxiv, Cold Spring Harbor Laboratory, 2021


Affinity Series of Genetically Encoded Förster Resonance Energy-Transfer Sensors for Sucrose


Mayuri Sadoine, Mira Reger, Ka Man Wong, Wolf B Frommer


ACS sensors, ACS Publications, 2021


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Projects


A success story


Project leads at ICIB- “If you want to go fast, go alone. If you want to go far, go together”


Watching at the thieves


Project lead at IMP - "Keep your friends close and your enemies closer"


Click chemistry & smFRET


Work made at FZJ, RWTH Aachen and HHU


Fluorescent biosensors


Work made at Carnegie Institution for Science, FZJ & HHU- "Seeing is believing"

Lecture


Genetically encoded fluorophore based sensor engineering

Lecture made at HHU (for IMP, PI: Wolf B. Frommer).


Fluorimetric analyses using Tecan plate readers

Workshop made at HHU (for IMP, PI: Wolf B. Frommer).

What else


About sensors

"Seeing is believing"


Advice to my younger self

"No one can make you feel inferior without your consent."- Eleanor Roosevelt


Advocacy & Mentoring

“If I have seen further it is by standing on the shoulders of giants.” — Isaac Newton


Community service

"Strive not to be a success, but rather to be of value." – Albert Einstein


Fights I support

"There are many causes that I am prepared to die for, but no causes that I am prepared to kill for"- Mahatma Gandhi


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