Mayuri Sadoine

Biophysicist & Microbiologist Making Biosensors for Studying Plant-Microbe Systems

Curriculum vitae

"I never lose. I either win or learn" -Nelson Mandela

Department of Biology

Heinrich Heine Universität

Universitätstrasse 1, 40225 Düsseldorf (Germany)

Mayuri Sadoine

Biophysicist & Microbiologist Making Biosensors for Studying Plant-Microbe Systems


Mayuri Sadoine

Biophysicist & Microbiologist Making Biosensors for Studying Plant-Microbe Systems

Curriculum vitae

"I never lose. I either win or learn" -Nelson Mandela

Department of Biology

Heinrich Heine Universität

Universitätstrasse 1, 40225 Düsseldorf (Germany)

About me

In summary: I am a biologist specializing in molecular biophysics, biosensor engineering, single-molecule techniques, and microbiology. My expertise spans the study of pathogens and beneficial microbes, particularly in plant-microbe interactions. My passion for science has led me to pursue this as my dream career. My research focus into understanding plant-microbe dynamics, exploring why microbes adopt a pathogenic or beneficial lifestyle, and unraveling the mechanisms behind their successful colonization of host organisms and interactions with them. I'm particularly intrigued by the evolutionary forces driving microbe behavior, whether it be egoistic or mutualistic.

My work necessitates a deep dive into host-microbe communications, requiring me to be an observer within the system. To achieve this, I use  fluorophore-based biosensors combined with advanced imaging techniques and microfluidics. These tools allow me to track and analyze microbial activities within their host environments effectively and to address further questions using various omics technologies.

In more detail: Throughout my PhD at Juelich Research Center and subsequent postdoc at HHU, I honed my skills in molecular biophysics and biosensor engineering. Specifically, I focused on developing genetically encoded biosensors capable of measuring analyte levels in vivo within specific organisms. These biosensors serve as invaluable tools for unraveling intricate biological processes, such as cellular responses to environmental cues."

Beyond research, I am deeply committed to advancing diversity and equity in STEM fields through active engagement in teaching, mentoring, and advocacy. On this website, you can have further into my journey, research interests, and the initiatives I support.

Currently, I am getting a closer look into the intricate questions of bacterial plant-microbe interactions, encompassing both beneficial and pathogenic strains.  Therefore, my focus lies in unraveling how microbes interact with the host plants. At the core of our research are hypotheses based on the idea that microbes colonize hosts to create optimal conditions for their multiplication. For bacterial plant-microbes, multiplication is intricately tied to nutrition. Thus, my research centers on how these unicellular organisms acquire nutrients and establish communication networks, both amongst themselves and with the host, to facilitate colonization, nutrition and multiplication within the unique environment of the host plant.

In many cases, the survival and multiplication of microbes lie on their optimal manipulation of host resources and their ability to outpace host defenses in a continuous race for dominance. Understanding these host-microbe interactions requires the ability to immerse oneself as an observer within the system—an endeavor that is as fascinating as it is challenging.

To this end, I employ biosensors and microfluidic devices  designed to detect changes in steady-state levels of molecules associated with nutrient transport, metabolism, and signaling within the microbe and the plant. By doing so, I aim to able to get better insights into the feeding habits and communication strategies of microbes within plant tissues and environment, as well as uncover the molecular mechanisms that underpin their success. This is possible by using an integrative approach which combined my expertise with different omics technologies.

The insights gained from studying microbe-host interactions are applicable across a spectrum of host-microbe systems. For example, evolutionary mechanisms driving virulence in pathogens mirror those found in various infectious agents, all of which must navigate the delicate balance of co-evolution with hosts while evading detection by the host's defense mechanisms.

In my research, I have, among others, worked on pathogens known to cause significant epidemiological challenges in crops, particularly in regions where these crops are vital for sustenance and economic stability. The resulting crop losses can have profound implications for local populations, often exacerbating food insecurity and public health concerns. Consequently, our research endeavors are underscored by a dual imperative: providing short-term solutions to crisis situations, such as developing tools for farmers, while simultaneously working towards long-term sustainability through the cultivation of resistant crop varieties and a deeper understanding of the factors influencing pathogen virulence. This holistic approach is essential for anticipating and mitigating the emergence of new pathogenic strains, drawing valuable insights from past epidemiological events.                     


A monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes shift fluorescent protein

Jona Obinna Ejike, Mayuri Sadoine, Yi Shen, Yuuma Ishikawa, Erdem Sunal, Sebastian Haensch, Anna B Hamacher, Wolf B Frommer, Michael M Wudick, Robert E Campbell, Thomas J Kleist

Biochemistry, ACS Publications, 2023

Monitoring nutrients in plants with genetically encoded sensors: Achievements and perspectives

Mayuri Sadoine, Roberto De Michele, Milan Župunski, Guido Grossmann, Vanessa Castro-Rodríguez

Plant Physiology, Oxford Academic Journals, 2023

OzTracs: Optical Osmolality Reporters Engineered from Mechanosensitive Ion Channels

Thomas J. Kleist, I Winnie Lin, Sophia Xu, Grigory Maksaev, Mayuri Sadoine, Elizabeth S. Haswell, Wolf B. Frommer, Michael M. Wudick

Biomolecules, vol. 12(6), 2022, p. 787

Sensors for the quantification, localization and analysis of the dynamics of plant hormones

Reika Isoda, Akira Yoshinari, Yuuma Ishikawa, Mayuri Sadoine, Rüdiger Simon, Wolf B Frommer, Masayoshi Nakamura

The Plant Journal, vol. 105, Wiley Online Library, 2021, pp. 542--557

Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology

Mayuri Sadoine, Yuuma Ishikawa, Thomas J Kleist, Michael M Wudick, Masayoshi Nakamura, Guido Grossmann, Wolf B Frommer, Cheng-Hsun Ho

Plant Physiology, Oxford Academic Journals, 2021

Affinity Series of Genetically Encoded Förster Resonance Energy-Transfer Sensors for Sucrose

Mayuri Sadoine, Mira Reger, Ka Man Wong, Wolf B Frommer

ACS sensors, ACS Publications, 2021

Affinity Purification of GO-Matryoshka Biosensors from E. coli for Quantitative Ratiometric Fluorescence Analyses

Mayuri Sadoine, Vanessa Castro-Rodriguez, Tobias Poloczek, Helene Javot, Erdem Sunal, Michael M Wudick, Wolf B Frommer

Bio-protocol, vol. 10, 2020, pp. e3773--e3773

Preparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies

Mayuri Sadoine, Michele Cerminara, Jörg Fitter, Alexandros Katranidis

Bio-protocol, vol. 8, 2018, pp. e2881--e2881

Cotranslational incorporation into proteins of a fluorophore suitable for smFRET studies

Mayuri Sadoine, Michele Cerminara, Michael Gerrits, Jörg Fitter, Alexandros Katranidis

ACS synthetic biology, vol. 7, ACS Publications, 2018, pp. 405--411

Selective double-labeling of cell-free synthesized proteins for more accurate smFRET studies

Mayuri Sadoine, Michele Cerminara, Noémie Kempf, Michael Gerrits, Jörg Fitter, Alexandros Katranidis

Analytical chemistry, vol. 89, ACS Publications, 2017, pp. 11278--11285

Cell-Free Synthesis of Site-Specifically Double-Labeled Proteins for More Accurate Single-Molecule FRET Studies

Mayuri Sadoine, Michele Cerminara, Noemie Kempf, Alexandros Katranidis, Jörg Fitter

Biophysical Journal, vol. 112, Elsevier, 2017, pp. 31a

Differential regulation of glucose transport activity in yeast by specific cAMP signatures

Clara Bermejo, Farzad Haerizadeh, Mayuri Sadoine, Diane Chermak, Wolf B Frommer

Biochemical Journal, vol. 452, Portland Press Ltd., 2013, pp. 489--497


Illuminating plant-microbe interactions with using biosensors

“If you want to go fast, go alone. If you want to go far, go together”

A success story: watching at the thieves

"Keep your friends close and your enemies closer"

Biosensors, Click chemistry & smFRET

"Seeing is beleiving"


Bachelor thesis: BSc. Celine Weippert

Second examiner/reviewer of the thesis entitled: Visualization of calcium signaling in Bacillus rhizobacteria during Arabidopsis thaliana root colonization using genetically encoded fluorescent biosensors.

Bachelor thesis: BSc. Clelia Sara Lotito

Second examiner/reviewer of the thesis entitled : Visualization and comparison of stress-induced calcium signaling in Arabidopsis thaliana roots and leaves using genetically encoded fluorescence-based biosensors.

Master thesis: MSc. Alfonso Gamaza Vico

External Professional/co-tutor of the thesis entitled: Fluo-resce in the face of adversity: Design and use of sensors to understand Nitrogen (N) transport in plants

CEPLAS undergraduate internship: BSc. Celine Weippert

Second examiner/reviewer of the thesis entitled: : Genomic integration and imaging of a fluorophore based GA-MatryoshCaMP6s sensor in Bacillus subtilis strain DB430.

M4457 MSc module: Optogenetic Cell Control, Advanced Microscopy and Quantitative Imaging

Conceived and independently supervised parts of this Module (for ICIB PI: Prof. Dr. Guido Grossmanss)

M4459 MSc Module: Biosensor Engineering: Principles and Strategies

Conceived and independently supervised parts of this Module (for ICIB, PI: Prof. Dr. Guido Grossmanss)

Master thesis: MSc. Erdem Sunal

Co-supervisor/advisor of the thesis entitled: Monitoring glucose dynamics in a plant pathogen by using genetically encoded fluorophore-based biosensors.

Genetically encoded fluorophore based sensor engineering

Lecture made at HHU (for IMP, PI: Wolf B. Frommer).

Fluorimetric analyses using Tecan plate readers

Workshop made at HHU (for IMP, PI: Wolf B. Frommer).

What else

About sensors

"Seeing is believing"

Advice to my younger self

"No one can make you feel inferior without your consent."- Eleanor Roosevelt

Advocacy & Mentoring

“If I have seen further it is by standing on the shoulders of giants.” — Isaac Newton

Being singular...

" have to be singular, inflexible, unyielding in your own work so that even the struggle, that very struggle to achieve becomes its own reward"- Martin Scorsese

Community service

"Strive not to be a success, but rather to be of value." – Albert Einstein

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